翻訳と辞書 Words near each other ・ Single-Level Dome ・ Single-level store ・ Single-line ・ Single-line working ・ Single-linkage clustering ・ Single-lip embouchure ・ Single-loss expectancy ・ Single-machine scheduling ・ Single-member district ・ Single-minded Dandelion ・ Single-Minute Exchange of Die ・ Single-mode optical fiber ・ Single-molecule experiment ・ Single-molecule FRET ・ Single-molecule magnet ・ Single-molecule magnetic sequencing ・ Single-nucleotide polymorphism ・ Single-O ・ Single-occupancy vehicle ・ Single-occupant vehicle ・ Single-origin coffee ・ Single-page application ・ Single-pair ・ Single-pair high-speed digital subscriber line ・ Single-parent homeschooling ・ Single-particle spectrum ・ Single-party period of the Republic of Turkey ・ Single-party state ・ Single-pass bore finishing ・ Single-payer health care Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Single-molecule magnetic sequencing ： ウィキペディア英語版 Single-molecule magnetic sequencing Magnetic sequencing is a single-molecule sequencing method in development. A DNA hairpin, containing the sequence of interest, is bound between a magnetic bead and a glass surface. A magnetic field is applied to stretch the hairpin open into single strands, and the hairpin refolds after decreasing of the magnetic field. The hairpin length can be determined by direct imaging of the diffraction rings of the magnetic beads using a simple microscope. The DNA sequences are determined by measuring the changes in the hairpin length following successful hybridization of complementary nucleotides. == Single-molecule sequencing vs. Next-generation sequencing == With the development of various next-generation sequencing platforms, there has been a substantial reduction in costs, and increase in throughput of DNA sequencing. However, the majority of the sequencing technologies rely on PCR-based clonal amplification of the DNA molecule in order to bring the signal to a detectable range. Sequencing of amplified clusters, or bulk sequencing in such a propose a read length-dependent phasing problem. During each cycle, not all of the molecules within the bulk have successful incorporation of an additional nucleotide. With increased sequencing cycle, the signal of the lagging molecules will eventually overwhelm the true signal. The phasing problem is a major limitation for the read lengths of the next-generation sequencing technologies. Therefore, there is an increased interest in developing single-molecule sequencing technologies, where no amplification is required. This not only shortens the preparation time for the sequencing libraries, it also has the potential to achieve much longer read lengths, as the lagging molecules with failed extensions can be ignored or considered separately. Previously known single-molecule sequencing technologies include Nanopore sequencing (Oxford Nanopore), SMRT sequencing (Pacific Biosciences), and Heliscope single molecule sequencing (Helicos Biosciences). 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Single-molecule magnetic sequencing」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.